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Karthick Raja Namasivayam, S.
- Evaluation of Lignocellulosic Agro Wastes for the Enhanced Production of Extracellular Cellulase and Xylanase by Trichoderma harzianum
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Authors
Affiliations
1 Department of Biotechnology, Sathyabama University, Chennai 600 119, T.N., IN
2 Department of Chemical Engineering, Sathyabama University, Chennai 600 119, T.N., IN
1 Department of Biotechnology, Sathyabama University, Chennai 600 119, T.N., IN
2 Department of Chemical Engineering, Sathyabama University, Chennai 600 119, T.N., IN
Source
Nature Environment and Pollution Technology, Vol 14, No 1 (2015), Pagination: 47-52Abstract
In the present study, various lignocellulotic agro-wastes paddy straw, coir pith, sugarcane bagassae and dried leaf litter were evaluated for cellulase and xylanase enzyme production adopting solid state fermentation with different moisture levels ranging from 0 to 50% by soil isolate of Trichoderma harzianum. The crude enzyme obtained was partially purified by ammonium sulphate precipitation and DEAE cellulose column chromatography. All the wastes supported growth and enzyme production. Maximum cellulase and xylanase production was recorded in paddy straw followed by sugarcane bagassae with 25% moisture content. Both the enzymes retained maximum activity at 60°C and pH 8.0. The partially purified enzyme by ammonium sulphate precipitation at 60% saturation followed by DEAE cellulose column chromatography yields pure xylanase with the molecular weight of 56 and 61 KD.Keywords
Agro Wastes, Trichoderma harzianum, Cellulase, Xylanase, Enzyme Activity.Full Text
- Biocompatible Formulation of Potential Fungal Biopesticide Nomuraea rileyi (f.) Samson for the Improved Post Treatment Persistence and Biocontrol Potential
Abstract Views :92 |
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Authors
Affiliations
1 Department of Biotechnology, Sathyabama University, Chennai, Tamil Nadu, IN
1 Department of Biotechnology, Sathyabama University, Chennai, Tamil Nadu, IN
Source
Nature Environment and Pollution Technology, Vol 13, No 4 (2014), Pagination: 835-838Abstract
The development of pest control measures using microorganisms, especially entomopathogens, has received increasing attention in recent years. Formulation of biological control agent is an important criterion for sustainable agriculture. Fungal biopesticides mainly Nomuraea rileyi is widely used to control various economic important insect pests. In the present study, various formulations of N. rileyi biogel, oil and hydrogel were prepared, and evaluated for post treatment persistence under different temperatures and the biocontrol potential against groundnut defoliator Spodoptera litura (Fab.) (Lepidoptera Noctuidae). Among the formulations, maximum rate of persistence was recorded in biogel. Fungal spores could retain the viability in all the tested temperatures in biogel formulation. Enhanced pesticidal activity was also recorded in the same formulation. The present study suggests the possible utilization of biogel formulation of N. rileyi to control economic important pests under field conditions.Keywords
Fungal Biopesticide, Nomuraea rileyi, Insect Pests, Biogel.Full Text
- Biocompatible Formulation of Probiotic Bacteria Bacillus subtilis with Agriculture Based Products for the Effective Acid Tolerance Properties and Synergistic Activity with Antibiotics
Abstract Views :227 |
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Authors
Affiliations
1 Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Old Mamallapuram Road, Sholinganallur, Chennai 600 119, Tamil Nadu, IN
2 Department of Chemical Engineering, Sathyabama University, Chennai 600119, IN
3 Centre for Bioresource and Development (C-BIRD), Department of Biotechnology, Sathyabama University, Chennai 600119, Tamil Nadu, IN
1 Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Old Mamallapuram Road, Sholinganallur, Chennai 600 119, Tamil Nadu, IN
2 Department of Chemical Engineering, Sathyabama University, Chennai 600119, IN
3 Centre for Bioresource and Development (C-BIRD), Department of Biotechnology, Sathyabama University, Chennai 600119, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 11, No 1 (2018), Pagination: 73-78Abstract
The present study was aimed to evaluate the acid tolerance properties and compatibility with antibacterial antibiotics of probiotic bacterial strain Bacillus subtilis formulated with various agriculture based products like potato peel and biogel. Bacterial strain was isolated from butter milk and the isolated probiotic strain was identified based on morphological, cultural and molecular characteristics which reveals the isolated strain belong to B.subtilis and the pure culture was formulated with the respective agriculture based products under aseptic condition. Acid tolerance study was carried out by determination of growth of respective formulation under different pH levels (6.5,4.5,2.5) which shows all the tested formulation retained the maximum viability in all the tested pH under all the incubation time. Compatibility of respective formulation with antibiotics was also investigated by measuring the zone of inhibition. Growth inhibition was not recorded in the majority of the antibiotics which reveals the best compatibility of formulation with the antibiotics which would suggests the possible utilization of formulated B.subtilis as an effective probiotic strain.Keywords
Bacillus subtilis, Probiotics, Formulation, Acid Tolerance, Compatibility, Antibiotics.
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